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α-Synuclein Aggregation Intermediates form Fibril Polymorphs with Distinct Prion-like Properties
Journal
Journal of Molecular Biology
ISSN
00222836
Date Issued
2022-10-15
Author(s)
Mehra, Surabhi
Ahlawat, Sahil
Kumar, Harish
Datta, Debalina
Navalkar, Ambuja
Singh, Nitu
Patel, Komal
Gadhe, Laxmikant
Kadu, Pradeep
Kumar, Rakesh
Jha, Narendra N.
Sakunthala, Arunima
Sawner, Ajay S.
Padinhateeri, Ranjith
Udgaonkar, Jayant B.
Agarwal, Vipin
Maji, Samir K.
Abstract
α-Synuclein (α-Syn) amyloids in synucleinopathies are suggested to be structurally and functionally diverse, reminiscent of prion-like strains. The mechanism of how the aggregation of the same precursor protein results in the formation of fibril polymorphs remains elusive. Here, we demonstrate the structure–function relationship of two polymorphs, pre-matured fibrils (PMFs) and helix-matured fibrils (HMFs), based on α-Syn aggregation intermediates. These polymorphs display the structural differences as demonstrated by solid-state NMR and mass spectrometry studies and also possess different cellular activities such as seeding, internalization, and cell-to-cell transfer of aggregates. HMFs, with a compact core structure, exhibit low seeding potency but readily internalize and transfer from one cell to another. The less structured PMFs lack transcellular transfer ability but induce abundant α-Syn pathology and trigger the formation of aggresomes in cells. Overall, the study highlights that the conformational heterogeneity in the aggregation pathway may lead to fibril polymorphs with distinct prion-like behavior.
Volume
434
Subjects