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Mass spectrometry and proteome analysis to identify SARS-CoV-2 protein from COVID-19 patient swab samples
Journal
STAR Protocols
Date Issued
2022-03-18
Author(s)
Banerjee, Arghya
Pai, Medha Gayathri J.
Singh, Avinash
Nissa, Mehar Un
Srivastava, Sanjeeva
Abstract
With new emerging SARS-CoV-2 strains and their increased pathogenicity, diagnosis has become more challenging. Molecular diagnosis often involves the use of nasopharyngeal swabs and subsequent real-time PCR-based tests. Although this test is the gold standard, it has several limitations; therefore, more complementary assays are required. This protocol describes how to identify SARS-CoV-2 protein from patients' nasopharyngeal swab samples. We first introduce the approach of label-free quantitative proteomics. We then detail target verification by triple quadrupole mass spectrometry (MS)-based targeted proteomics. For complete details on the use and execution of this profile, please refer to Bankar et al. (2021).
Subjects