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Monitoring the Disruptive Effects of Tubulin-Binding Agents on Cellular Microtubules
Journal
Methods in Molecular Biology
ISSN
10643745
Date Issued
2022-01-01
Author(s)
Kumari, Anuradha
Panda, Dulal
Abstract
Tubulin-binding agents are an important class of chemotherapeutic agents. This chapter describes detailed protocols to examine the effects of tubulin-binding agents on cellular microtubules. The methods can be utilized for the screening of novel chemotherapeutic agents targeting microtubules. These assays can also be extended to study the effects of various proteins on the stability of microtubules. We have described five assays, which together provides qualitative and quantitative information about the effects of tubulin-binding agents on microtubule stability and dynamics. The key steps and crucial information regarding different steps have been included along with the theory of each of the assays.
Volume
2430
Subjects